Part:BBa_K2971000:Design
crtE from Deinococcus radiodurans
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 841
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 43
Design Notes
Codon optimzed for expression E.coli and to make it biobrick compatible.
The gene was selected from Deinococcus radiodurans due to the organism’s extremophilic properties. D.radiodurans exhibit several traits that would make it ideal in our imagined biogenic solar cell (see our design page), such as a high resistance towards ionizing radiation and dehydration. Although the translated protein does not necessarily exhibit the same resistances as the whole organism, it was selected to highlight our interest in D.radiodurans. The gene was placed in an expression vector under pBAD induction so that we could overexpress the protein to test its enzymatic activity. Due to time restraints, and the general difficulty of characterizing carotenogenic enzymes, this was not achieved.
Source
Deinococcus radiodurans.